[Objectives]To investigate the effects of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama.[Methods]Taking the normal feeding group at 26 ℃ as the control,the expression change of AaHsp90 was detected by real-time PCR in midgut,fat body and hemlymph after high temperature stress at 38 ℃,low temperature stress at 4 ℃ and starvation stress separately for different time on the third day of the fifth larvae.[Results]The expression of AaHsp90 in midgut,fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38 ℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut,fat body and hemolymph after treatment with 4 ℃ for different time.After treatment with starvation,the AaHsp90 expression were increased at 12 and 18 h and decreased sharply at 24 h in midgut,fat body and hemolymph of A. assama.[Conclusions]Comprehensive analysis showed that high temperature and starvation stress can induce the expression of AaHsp90,while low temperature stress mainly suppressed its expression.It was suggested that the AaHsp90 protein may play an important role in the process of adaptation to high temperature and starvation stress in A. assama.