Given the importance of knowledge-based firms in the agricultural sector, this paper aimed to identify barriers hindering companies from becoming knowledge-based in the dominant competitive and dynamic environment. To this end, using a qualitative approach, in-depth interviews were conducted to collect data from 38 experts and managers in agricultural companies working in Alborz Science and Technology Park Isfahan, Hamedan, and Tehran University. The main criterion for determining the sample size was to reach the theoretical saturation. All respondents were selected using a theoretical and purposeful sampling method. Data coding and analysis procedures were completed in three stages. According to the results of the study, five main internal and external elements were identified: market inefficiency, governance-administrative barriers, inefficient union or organization, lack of skills and professionalism, and the internal inefficiency of the company.