Fresh blueberries are sold through a marketing cooperative of the blueberry industry in Mississippi and Louisiana. Blueberry producers have numerous alternatives in assembling blueberries, and the cooperative needs to know the costs of different systems for assembling berries in order to provide better services to its members. The main objective of this study was to determine an efficient system for handling blueberries in Mississippi and Louisiana. Sixteen models with different combinations of drop stations and shipping points were evaluated. The results of this study will help the Miss-Lou Association and farmers to better develop strategies for handling and marketing blueberries in the future. It will assist the Miss-Lou Association in determining the number, size, and locations of drop stations/shipping points with change in production each year.