The aim of this paper is to identify the motives of the decisions to join the agritourism business in the Czech Republic and the degree of fulfilment of these input motives. The research also identified the differences in motives for the entry of farmers into agritourism in the Czech Republic as compared to the results of a study on agritourism in the USA. In order to achieve the aims, descriptive statistic methods and non-parameterized testing through Wilcoxon test were used. It was found out that unlike the USA, in the Czech Republic the most dominant motives for joining agritourism are economic motives. The results also included a comparison of the approaches reflecting the impact of existing experience with agritourism with the change of approaches to the input motives. The article originated as a part of the Internal Grant Agency (IGA) of the Czech University of Life Sciences in Prague, Registration Number 20121074.